TABLE 2.

C_T values of the A(H3N2)-positive clinical specimens given by two real-time RT-PCR assays

Specimen no.	CT values from the indicated assay		ΔCTc	HA clade [A(H3N2)]	Collection date (in 2013)
	Aw1a	Atb
95,298	29.34	23.74	5.60	TW2011-13	9 Octoberd
95,370	29.19	23.92	5.27	TW2011-13	2 March
95,537	35.68	25.08	10.60	TW2011-13	8 May
95,638	38.98	26.24	12.74	TW2011-13	6 July
95,666	35.33	24.33	11.00	TW2011-13	16 August
95,679	UDe	30.89		TW2011-13	23 August
80,068	30.72	25.81	4.91	TW2011-13	24 January
80,158	28.29	23.05	5.24	TW2011-13	18 February
80,159	29.09	23.72	5.37	TW2011-13	18 February
80,162	25.01	20.22	4.79	TW2011-13	18 February
80,222	33.45	22.64	10.81	TW2011-13	2 March
80,225	32.09	24.05	8.04	TW2011-13	4 March
80,710	31.35	21.16	10.19	TW2011-13	10 May
80,720	32.17	20.79	11.38	TW2011-13	13 May
80,723	35.80	26.79	9.01	TW2011-13	14 May
80,231	UD	33.21		NDf	5 March
80,270	UD	34.54		ND	13 March
80,636	35.35	33.11	2.24	ND	1 May
80,665	UD	36.15		ND	5 May
80,683	36.54	29.56	6.98	TW2011-13	7 May
80,864	31.77	23.71	8.06	TW2011-13	3 June
80,889	37.31	28.09	9.22	TW2011-13	July 6
80,911	37.90	29.41	8.49	TW2011-13	10 June
80,950	35.04	27.02	8.02	TW2011-13	15 June
80,978	24.52	20.57	3.95	TW2011-13	22 June
81,007	36.95	29.09	7.86	TW2011-13	29 June
81,013	UD	27.52		TW2011-13	28 June
81,018	21.88	19.12	2.76	TW2011-13	1 July
81,024	UD	32.54		ND	3 July
81,083	37.23	27.69	9.54	TW2011-13	22 July
81,111	UD	31.32		ND	4 August
81,130	34.31	22.58	11.73	TW2011-13	13 August
81,139	32.68	21.86	10.82	TW2011-13	16 August
81,156	39.78	29.33	10.45	TW2011-13	23 August
77,163	38.67	27.00	11.67	TW2011-13	1 May
77,917	35.08	24.27	10.81	TW2011-13	16 July
78,001	37.90	29.29	8.61	TW2011-13	29 July
78,056	UD	36.00		ND	3 August
78,093	UD	34.61		ND	13 August
78,128	UD	28.93		TW2011-13	19 August

^aAssay recommended by the WHO for the detection of all influenza A viruses.

^bModified assay established in this study.

^cValues = (C_T of Aw1) − (C_T of At).

^dIn 2012.

^eUD, M gene of the influenza A virus was undetectable.

^fND, unavailable.