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. 2014 Jan;52(1):339–343. doi: 10.1128/JCM.00390-13

TABLE 2.

Nucleotide differences between AIP981.10 gene sequences and corresponding sequences from C. botulinum C or D and C/D mosaic, C. haemolyticum, and C. novyi NT

AIP981.10 gene/product (source) Size (bp) No. of nucleotide differences between AIP981.10 and corresponding Clostridium sequences
C. botulinum D/C Japanese bovine strain group C. botulinum C/D mosaic avian strains C. botulinum C-C. botulinum D-C. novyi B-C. haemolyticum C. botulinum C strain Eklund C. novyi NT
CTPs (PCR amplicon) 466 0 10 19 49 50
glpK (PCR amplicon) 579 0 9/121a 23 35/121a 56
rpoB (PCR amplicon) 627 0 6 23 49 56
gyrA (PCR amplicon) 558 0 26 29 72 82
dnaK (PCR amplicon) 525 0 11/217a 15 33 24/213a
Botulinolysin (PCR amplicon) 902 2 26/154a 176 168/195a 210
Phospholipase C (gene sequence from WGS) 1,200b NA 55/234a 74/77/NA/105c 388 394
C3 exoenzyme (gene sequence from WGS) 735b NA 16 16/1/NA/NAd 11 NA
a

Some genomes show two gene copies of dnaK, glpK, and the botulinolysin and phospholipase C genes that are distantly related. (Differences in both gene copies are separated by a slash.) The corresponding GenBank accession numbers of these genomes are listed in Table S2 in the supplemental material.

b

Data obtained from the whole-genome sequencing (WGS) of strain AIP981.10.

c

Nucleotide difference between the phospholipase C gene from AIP981.10 and those from C. botulinum C (AB699605), C. botulinum D (AB699604), C. novyi B (not available [NA]), and C. haemolyticum (AF525415), respectively.

d

Nucleotide difference between the C3 exoenzyme gene from AIP981.10 and those from C. botulinum C (X59039), C. botulinum D (D17555), C. novyi B (NA), and C. haemolyticum (NA), respectively.