TABLE 2.
Modification of identification level by species in 71 specimens
| Species (no. of samples) | Conventional method result | No. (%) of samples with the indicated result by conventional methods with SDS pretreatmentd |
No. of samples (%) with whole identification improvementa | |||
|---|---|---|---|---|---|---|
| No peaks found | ID not reliable | ID genus level | ID species level | |||
| E. coli (34) | No peaks found | 13 (38.2)c | 1(2.9)c | 5 (14.7)b | 10 (29.4)b | 19 (55.9) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 0 | 0 | 1 (2.9)c | 4 (11.8)b | ||
| E. faecalis (16) | No peaks found | 10 (62.5)c | 0 | 1 (6.3)b | 2 (12.5)b | 6 (37.6) |
| ID not reliable | 0 | 0 | 1 (6.3)b | 2 (12.5)b | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| Pseudomonas aeruginosa (4) | No peaks found | 0 | 0 | 1 (25)b | 2 (50)b | 4 (100) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 0 | 0 | 0 | 1 (25)b | ||
| Streptococcus agalactiae (3) | No peaks found | 1 (33.3)c | 0 | 0 | 0 | 0 (0) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 1 (33.3)c | 1 (33.3)c | 0 | 0 | ||
| K. pneumoniae (2) | No peaks found | 0 | 1 (50)c | 0 | 1 (50)b | 1 (50) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| Staphylococcus aureus (2) | No peaks found | 0 | 0 | 0 | 0 | 1 (50) |
| ID not reliable | 1 (50%)c | 0 | 0 | 1 (50)b | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| Staphylococcus saprophyticus (2) | No peaks found | 2 (100)c | 0 | 0 | 0 | 0 (0) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| Providencia stuartii (1) | No peaks found | 0 | 0 | 0 | 0 | 0 (0) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 1 (100)c | 0 | 0 | 0 | ||
| Raoultella ornithinolytica (1) | No peaks found | 0 | 0 | 0 | 0 | 0 (0) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 0 | 0 | 1 (100)c | 0 | ||
| Salmonella species (1) | No peaks found | 0 | 0 | 0 | 0 | 1 (100) |
| ID not reliable | 0 | 0 | 1 (100)b | 0 | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| Klebsiella oxytoca (1) | No peaks found | 1 (100)c | 0 | 0 | 0 | 0 (0) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| Enterobacter cloacae (1) | No peaks found | 1 (100)c | 0 | 0 | 0 | 0 (0) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| P. mirabilis (1) | No peaks found | 0 | 0 | 0 | 0 | 0 (0) |
| ID not reliable | 0 | 1 (100)c | 0 | 0 | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| Enterococcus faecium (1) | No peaks found | 0 | 0 | 0 | 1 (100)b | 1 (100) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| Streptococcus pyogenes (1) | No peaks found | 1 (100)c | 0 | 0 | 0 | 0 (0) |
| ID not reliable | 0 | 0 | 0 | 0 | ||
| ID genus level | 0 | 0 | 0 | 0 | ||
| Gram-negative bacteria | No peaks found | 15 (41.7)c | 2 (5.6)c | 6 (16.7)b | 13 (36.1)b | 25 (54.3) |
| ID not reliable | 0 | 1 (50)c | 1 (50)b | 0 | ||
| ID genus level | 1 (12.5)c | 0 | 2 (25)c | 5 (62.5)b | ||
| Gram-positive bacteria | No peaks found | 14 (77.8)c | 0 | 1 (5.6)b | 3 (16.7)b | 8 (32) |
| ID not reliable | 1 (20)c | 0 | 1 (20)b | 3 (60)b | ||
| ID genus level | 1 (50)c | 1 (50)c | 0 | 0 | ||
| Total | 33 (46.5) | |||||
a
Proportion of samples increasing identification level. For this purpose, “no peaks found” and “ID not reliable” were considered as the same level.
b
ID level improves.
c
ID level does not improve.
d
“0” indicates no specimens in the category.