FIG 2.

The second and third lysines in cluster 3 regulate Ume6p repressor function. (A) A ume6Δ strain harboring plasmids expressing wild-type UME6 or the mutant derivatives, as indicated, was transformed with a spo13-lacZ reporter plasmid, and β-galactosidase activity was determined as described previously (65). Mean values from three independent biological replicates are presented (±standard errors of the means). (B) SPO13, INO1, and CAR1 mRNA levels were monitored by RT-qPCR from mid-logarithmic-phase UME6-, UME6^K3R-, or UME6^K3Q-expressing cultures. Mean values from three independent biological replicates are presented (±standard errors of the means) relative to ENO1 mRNA levels. Asterisks indicate statistically significantly differences from wild-type values as determined by the Student t test (P < 0.05).