TABLE 1.
Yeast strains used in this study
| Strain | Genotypea | Reference |
|---|---|---|
| RSY335 | MATa/α cyh2R-z ho::LYS2 leu2::hisG lys2 trp1::hisG ura3 | 18 |
| RSY1079 | T7-UME6 | 18 |
| RSY1091 | gcn5::TRP1 | This study |
| RSY1152 | ume6::leu2::KanMX | 18 |
| RSY1404 | gcn5::TRP1 ume6::leu2::KanMX | This study |
| RSY1407 | T7-UME6K3R | This study |
| RSY1408 | T7-UME6K3Q gcn5::TRP1 | This study |
| RSY1420 | T7-UME6K3Q | This study |
| RSY1500 | ADA2-TAP-his3MX6::LEU2 | This study |
| RSY1563 | ADA2-TAP-his3MX6::LEU2 gcn5::TRP1 | This study |
| RSY1632 | rpd3::TRP1 | This study |
| RSY1717 | MATα cyh2R-z ho::LYS2 leu2::hisG lys2 trp1::hisG ura3 T7-UME6K1R K3R | This study |
| RSY1718 | MATα cyh2R-z ho::LYS2 leu2::hisG lys2 trp1::hisG ura3 T7-UME6K1Q K3Q | This study |
| RSY1732 | MATα cyh2R-z ho::LYS2 leu2::hisG lys2 trp1::hisG ura3 T7-UME6K1R K3Q | This study |
| RSY1733 | MATα cyh2R-z ho::LYS2 leu2::hisG lys2 trp1::hisG ura3 T7-UME6K1Q K3R | This study |
| RSY1743 | T7-UME6K3R rpd3::TRP1 | This study |
| RSY1835 | MATa/α lys2 ura3 LYS2::hoΔ ume6::KANMX | This study |
a
All strains are isogenic to RSY335 except as noted. All alleles are homozygous except as noted.