TABLE 2.
Plasmids used in this study
| Plasmid | Genotype | Reference |
|---|---|---|
| pRS306 | YIp URA3 | 66 |
| pMM285 | T7-UME6 in pRS315 CEN6 LEU2 | 18 |
| pML2 | T7-UME6K812R,K813R,K814R in pMM285 | This study |
| pML3 | T7-UME6K812Q,K813Q,K814Q in pMM285 | This study |
| pML11 | T7-UME6K813R,K814R in pMM285 | This study |
| pML16 | T7-UME6K813Q,K814Q in pMM285 | This study |
| pML23 | T7-UME6K813R,K814R in pRS306 | This study |
| pML24 | T7-UME6K813Q,K814Q in pRS306 | This study |
| pMM288 | HIS6-UME6726–836 | This study |
| pML38 | HIS6-UME6K736R,K737R,K745R | This study |
| pMM293 | HIS6-UME6K812R,K813R,K814R | This study |
| pML40 | HIS6-UME6K736Q,K737Q,K745Q | This study |
| pML41 | HIS6-UME6K736R,K737R,K745R,K812R,K183R,K814R | This study |
| pML53 | HIS6-UME6K812Q,K813Q,K814Q | This study |
| pML63 | T7-Ume6p in pRS316; CEN6 URA3 | This study |
| pML64 | T7-Ume6pK3R in pRS316; CEN6 URA3 | This study |
| pML65 | T7-Ume6pK3Q in pRS316; CEN6 URA3 | This study |