TABLE 1.

Genes regulated by RSPO2 in C2C12 cells

Gene symbol	Gene product	Fold inductiona
		Microarray		qRT-PCR
		U1	D1	U1	D1
Axin2	Axin2	2.502	3.912	37.372	21.363
Car3	Carbonic anhydrase 3	2.489	2.81	ND	ND
Ch25h	Cholesterol 25-hydroxylase	3.075	2.207	ND	ND
Cxcr6	Chemokine (C-X-C motif) receptor 6	2.572	2.33	5.616b,c	6.755b,c
Enpp2	Ectonucleotide pyrophosphatase/phosphodiesterase 2	2.919	7.945	7.931	3.258
Fst	Follistatin	2.986	4.786	10.549	10.342
Il33	Interleukin 33	2.869	2.388	5.484b	4.303b
Lgr5	Leucine-rich repeat containing G protein-coupled receptor 5	3.677	4.444	51.921b	47.331b
Pfn2	Profilin 2	2.387	4.677	ND	ND
Qpct	Glutaminyl-peptide cyclotransferase (glutaminyl cyclase)	2.103	2.441	ND	ND
Tgfb2	Transforming growth factor beta 2	2.404	2.715	4.524b	3.173b
Tspan8	Tetraspanin 8	3.35	3.782	5.683	4.398
Aspn	Asporin	−2.447	−2.894	−2.583b	−1.877b
Cxcl12	Chemokine (C-X-C motif) ligand 12	−2.521	−2.254	−4.256b	−2.242b
Cxcl5	Chemokine (C-X-C motif) ligand 5	−2.414	−3.609	−14.507b	−21.22b
Enpp1	Ectonucleotide pyrophosphatase/phosphodiesterase 1	−2.531	−2.509	−2.699	−1.927
Enpp3	Ectonucleotide pyrophosphatase/phosphodiesterase 3	−2.241	−2.646	−2.061	−1.766
F3	Coagulation factor III	−2.736	−2.572	ND	ND
Fgf7	Fibroblast growth factor 7	−3.772	−3.745	−4.801b	−6.012b
Hgf	Hepatocyte growth factor	−2.784	−2.808	−2.916b,c	−2.673b
Lphn2	Latrophilin 2	−2.682	−2.367	ND	ND
Ptn	Pleiotrophin	−2.761	−3.871	−6.058b	−1.561b,d
Rgs2	Regulator of G protein signaling 2	−2.49	−4.442	−2.223	−1.7

^aP values of the fold induction of each gene are smaller than 0.01 except for the ones noted. U1, undifferentiated cells on day 1; D1, differentiated cells on day 1; ND, not determined.

^bqRT-CPR was performed using a customized RT² profiler PCR array from SA Biosciences. The names of primer sets are listed in Table S4 in the supplemental material.

^cP < 0.05.

^dNot statistically significant.