FIG 4.

Rescue of TRiC alleviates polyQ-expanded huntingtin aggregation. (A) HttQ103-GFP and Flag-VRK2 with or without HA-CCT4 were coexpressed in HEK293T cells, and fluorescence microscopic images were analyzed. Bar, 20 μm. (B) The percentage of cells with HttQ103-GFP aggregate foci was calculated. The error bars represent the mean ± SEM (n = 4). *, P < 0.05. (C) The amounts of HttQ103-GFP aggregates were investigated by Western blotting using an anti-GFP antibody. The insoluble aggregates remained at the top of the gel, and soluble HttQ103-GFP entered the resolving gel. (D) Filter-trap assay for analysis of the insoluble aggregates in cells transfected with the indicated vectors.