FIG 5.

Knockdown of VRK2 decreases formation of polyQ-expanded huntingtin aggregates. (A) HttQ103-GFP with or without siRNA against VRK2 was coexpressed in U2OS cells, and HttQ103 aggregates were investigated by immunoblotting. (B) Fluorescence microscopy of HttQ103-GFP expressed in HeLa cells treated with siVRK2 or siCont. VRK2 depletion decreased the number of cells containing HttQ103-GFP aggregates. Bar, 20 μm. (C) The percentage of aggregate-positive cells from panel B was calculated. The error bars represent the mean ± SD (n = 3). **, P < 0.01 versus siCont-treated cells at 2 and 3 days; n.s, not significant.