FIG 6.

The SULT2B1b-induced alleviation of insulin resistance may have been achieved by inhibiting the activity of HNF4α. An adenovirus encoding shHNF4α or the control, shLacZ, was introduced by tail vein injection into WT and TG mice that had been fed an HFD for 12 weeks. Mice were analyzed 1 week after infection. (A) HNF4α protein expression as shown by Western blotting. (B) Fasting blood glucose levels. (C) Relative hepatic expression of G6pase mRNA. (D and E) GTT (D) and ITT (E) results. The percentage in panel E is relative variation from the time 0 min, which was arbitrarily set as 100%. Quantifications of the areas under the curve (AUC) are shown on the right. Results are means ± SD; n, 4 mice per group. *, P < 0.05.