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. 2014 Feb;34(3):560–572. doi: 10.1128/MCB.00937-13

FIG 6.

FIG 6

Deletion of β1 integrin in the extraembryonic endoderm by Ttr-Cre and the epiblast by Sox2-Cre. E5.5 and E9.5 embryos were harvested from matings between male β1 integrin+/df; Ttr-Cre and female β1 integrinfl/fl mice. The E5.5 embryos were sectioned and stained for Dab2 (green), laminin (red), and DAPI (blue). The E9.5 embryos were dissected, photographed, and genotyped by PCR. Representative examples of β1 integrin-heterozygous (fl/df), Ttr-Cre-mediated deletion in extraembryonic endoderm and Sox2-Cre-mediated deletion in epiblast are shown. (A) Wild-type [Intb1 (fl/df)] E5.5 embryos were sectioned and stained with Dab2, laminin, and DAPI; E-cadherin, megalin, and DAPI; and Oct3/4, Dab2, and DAPI. (B) Mutant (β1 integrinfl/df; Ttr Cre) E5.5 embryos were sectioned and stained with Dab2, laminin, and DAPI and E-cadherin, megalin, and DAPI. (C) Mutant (β1 integrinfl/df; Sox2 Cre) E5.5 embryos were sectioned and stained with Dab2, laminin, and DAPI and Oct3/4, Dab2, and DAPI. (D) Representative E9.5 embryos of β1 integrinfl/df, β1 integrinfl/df; Ttr-Cre, and β1 integrinfl/df; Sox2-Cre types are shown.