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. 2014 Feb;34(3):325–334. doi: 10.1128/MCB.00912-13

FIG 6.

FIG 6

IRAK1 is required for LMP1-induced CaMKII activation and p65/RelA serine 536 phosphorylation. IRAK1 WT (lanes 1 to 4) or KO (lanes 5 and 6) MEFs were transfected with pSG5 (lanes 1 and 5), pSG5-FLAG-LMP1 WT (lanes 2 and 6), pSG5-FLAG-LMP1 1–231 (lanes 3 and 7), or pSG5-FLAG-LMP1 Δ187–351 (lanes 4 and 8). After 48 h, equal amounts of cell extracts were subjected to Western blot analysis with antibody to phospho-CaMKII threonine 286, phospho-p65/RelA serine 536, p65/RelA, CaMKII, tubulin, IRAK1, or FLAG. *, FLAG-LMP1 WT, CTAR1, or CTAR2. Additional nonspecific bands were detected, possibly due to a nonspecific binding of antibodies generated by insufficient blocking and/or washing of the membrane.