FIG 3.
Kinetic analysis of mitotic phosphorylation of eIF4G(Ser1232) in vivo. (A) The sequence of eIF4G(1229-1335) and quantitative computer kinase prediction (athe quantitative score is based on deduced consensus phosphorylation site amino acid frequency scoring matrices that have been determined for each of the ∼500 different human protein kinases [51]). (B) Kinetic analysis of eIF4G phosphorylation and cell cycle progression from G1/S to M phase. HEK293 cells were arrested near the G1/S boundary by Th block (4 mM, 9 h) and released with growth medium in the presence of 2 μM NOC. Mitotic cells were treated with APVL (10 μM, 1 h). Lysates were subjected to immunoblotting. The assay was performed for a minimum of three times, and a representative series is shown.