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. 2014 Feb;34(3):348–361. doi: 10.1128/MCB.00825-13

FIG 9.

FIG 9

Grb2 is required for FAK association with PTPα. (A) PTPα−/− MEFs were untreated or transfected as shown with control (Ctl) or Grb2 siRNA, myc-tagged Grb2-WT or the indicated Grb2 mutants, and VSVG-WT PTPα. VSVG immunoprecipitates from adherent cell lysates and cell lysates were probed as shown. (B) PTPα−/− MEFs were transfected with VSVG-WT-PTPα and VSVG-Y789F-PTPα. VSVG immunoprecipitates from adherent cell lysates and cell lysates were probed as shown. (C) FAK-null MEFs (FAK−/−) and FAK-null MEFs stably reexpressing GFP-tagged wild-type (FAK-WT) or the indicated mutant forms of FAK (Y397F or Y925F) were kept in suspension for 1 h (lanes 0) and replated on fibronectin (FN)-coated dishes for 15 min (lanes 15). Cell lysates were probed for the indicated (phospho)proteins.