FIG 5.

Neutralization of cell-free- and cell-to-cell-mediated infection. HIV-1-infected MDM and cell-free inocula were adjusted to produce equivalent infection levels at 48 h postinfection by the cell-to-cell (CTC; black bars) and cell-free (CF; open bars) routes for HIV-1_NL-LucR-BaL (A) and HIV-1_NL-LucR-YU2 (B). Means ± SEM are shown for 6 (BaL) and 4 (YU2) donors. The activity of bNAbs targeting the CD4bs (VRC01 and b12) and the glycan shield (PGT121 and 2G12) were tested against cell-to-cell (filled circles) and cell-free (open circles) infection of CD4⁺ T cells with HIV-1_{NL-LucR-T2A.BaL} (C) and HIV-1_{NL-LucR-T2A.YU2} (D). Means ± SEM from 3 to 6 donors are shown. The significance of differences in IC₅₀ was assessed by F test. A significant difference was observed for b12 (P = 0.002) against BaL. 2G12 was not tested against YU2.