FIG 1.

Lipoxygenase expression in human tissues and KSHV-infected cells. (A and B) Human KS and healthy skin tissue sections were analyzed by immunohistochemical staining for 5LO (A) and LTA4H (B) and counterstained with hematoxylin. (Aa, c, and e and Ba, c, and e) Healthy human skin tissue sections; (Ab, d, and f and Bb, d, and f) human KS skin tissue sections. Arrows (yellow) in all panels, 5LO or LTA4H staining. Magnifications: ×20 (Aa, b, e, and f, Ba, b, e, and f) and ×40 (Ac and d and Bc and d). (C) Immunofluorescence analysis of LANA-1 and 5LO in long-term KSHV latently infected ECs. TIVE and TIVE-LTC cells were grown to 80 to 90% confluence, fixed, permeabilized, and examined with LANA-1-specific (red) and 5LO-specific (green) antibodies. Nuclei were counterstained with DAPI (blue). Red arrows in panel Cb, nuclear staining for KSHV latency protein LANA-1; green arrows in panels Cc and i, 5LO staining; yellow arrows in panels Cd and j, LANA-1 and 5LO colocalization in nuclei; white arrows in panel Ce, k, f, and l, LANA-1 and 5LO colocalization with the nuclear stain DAPI. Magnifications: ×40 (Ca to k) and further enlargement by an additional ×40 (Cf and l).