FIG 5.

5LO and FLAP interaction during KSHV infection. (A, panel a) Uninfected endothelial (TIVE) cells, BJAB cells, KSHV-infected endothelial (TIVE-LTC) cells, or BCBL-1 cells were lysed and immunoprecipitated with anti-5LO and Western blotted (WB) with anti-5LO antibody. (A, panel b) TIVE, BJAB, TIVE-LTC, or BCBL-1 cells were lysed and immunoprecipitated with anti-5LO and Western blotted with anti-FLAP antibody. (B) BCBL-1 cells were immunostained with anti-lamin B (red) and anti-FLAP (green) or with 5LO (red) and FLAP (green) antibody and analyzed by confocal microscopy. Red arrows, nuclear membrane staining in panel 1 and nuclear localization of 5LO in panel 4; green arrows in panels 2 and 5, nuclear membrane localization of FLAP; yellow arrows, colocalization of FLAP with the nuclear membrane in panel 3 and colocalization of 5LO and FLAP in panel 6; white arrowheads in enlarged panels, colocalization of lamin B and FLAP and 5LO and FLAP.