FIG 9.

Effect of 5LO inhibition on KSHV infection-induced TH1/TH2-related cytokines in HMVEC-d. Supernatants collected from serum-starved (8 h) cells either uninfected or infected (4 h, 8 h, 24 h, and 48 h) or pretreated with either MK866, solvent for MK866, zileuton, or solvent for zileuton and then infected with KSHV for 4 h, 8 h, 24 h, and 48 h were used to analyze the levels of TH1- and TH2-related cytokines. Similarly, supernatants collected from serum-starved (8 h) sh-C- or sh-5LO-transduced HMVEC-d either uninfected or infected (4 h, 8 h, 24 h, and 48 h) were used to analyze the levels of TH1- and TH2-related cytokines. Fold induction was calculated by considering the secretion at 0 h infection to be 1-fold. Cytokine levels with respect to those of 0 h of infection are represented in a line graph format. (A) IFN-γ; (B) IL-2; (C) TNF-α; (D) IL-10; (E) IL-4; (F) IL-5.