TABLE 2.
Effect of 5LO pathway inhibition on TH2 cytokine secretion in uninfected and KSHV-infected cells at different time points
| TH2 cytokine, time, and infection statusa | Fold induction (% inhibition)b |
|||||
|---|---|---|---|---|---|---|
| MK sol. treat. | MK treat. | Zil. sol. treat. | Zil. treat. | sh-C | sh-5LO | |
| IL-10 | ||||||
| 4 h, uninf. | 1.2 | 1.1 (9) | 1.4 | 1.1 (22) | 1.4 | 0.9 (36) |
| 4 h, inf. | 1.8 | 1.3 (28) | 2.2 | 1.3 (51) | 2.8 | 1.4 (50) |
| 8 h, uninf. | 1.3 | 1.1 (16) | 1.3 | 1.2 (8) | 1.2 | 0.8 (34) |
| 8 h, inf. | 4.5 | 1.4 (60) | 4.3 | 1.6 (63) | 3.9 | 1.3 (66) |
| 24 h, uninf. | 1.6 | 0.9 (44) | 1.8 | 1.1 (39) | 1.9 | 1.1 (43) |
| 24 h, inf. | 5.4 | 1.8 (67) | 5.7 | 1.8 (69) | 5.5 | 1.4 (75) |
| 48 h, uninf. | 1.7 | 1.2 (30) | 1.7 | 1.1 (36) | 1.4 | 0.7 (50) |
| 48 h, inf. | 5.0 | 1.7 (66) | 5.2 | 1.7 (68) | 5.7 | 1.9 (66) |
| IL-4 | ||||||
| 4 h, uninf. | 1.2 | 1.1 (9) | 1.2 | 1.1 (9) | 1.3 | 0.8 (39) |
| 4 h, inf. | 1.5 | 1.5 | 1.8 | 1.8 | 1.9 | 1.7 (11) |
| 8 h, uninf. | 1.4 | 0.8 (43) | 1.3 | 1.1 (16) | 1.7 | 1.2 (30) |
| 8 h, inf. | 2.5 | 1.4 (44) | 2.5 | 1.4 (54) | 2.7 | 1.3 (52) |
| 24 h, uninf. | 1.4 | 1.2 (15) | 1.5 | 1.1 (17) | 1.5 | 1.1 (27) |
| 24 h, inf. | 2.3 | 1.1 (53) | 2.6 | 1.4 (46) | 2.3 | 1.4 (40) |
| 48 h, uninf. | 1.1 | 0.8 (28) | 1.2 | 0.8 (34) | 1.3 | 0.7 (47) |
| 48 h, inf. | 2.2 | 1.3 (41) | 2.4 | 1.3 (46) | 2.0 | 1.5 (25) |
| IL-5 | ||||||
| 4 h, uninf. | 1.2 | 0.8 (34) | 1.3 | 0.9 (31) | 1.3 | 0.9 (31) |
| 4 h, inf. | 2.0 | 2.3 | 2.2 | 2.3 | 2.4 | 2.2 (9) |
| 8 h, uninf. | 1.2 | 0.9 (25) | 1.3 | 1.1 (9) | 1.4 | 0.8 (43) |
| 8 h, inf. | 2.3 | 2.4 | 2.2 | 1.9 (14) | 2.2 | 2.1 (5) |
| 24 h, uninf. | 1.2 | 1.1 (9) | 1.5 | 0.9 (40) | 1.2 | 0.8 (34) |
| 24 h, inf. | 2.4 | 1.8 (25) | 2.2 | 1.8 (19) | 2.4 | 1.8 (25) |
| 48 h, uninf. | 1.3 | 1.1 (16) | 1.2 | 0.9 (25) | 1.2 | 0.8 (34) |
| 48 h, inf. | 2.0 | 1.7 (15) | 2.1 | 1.7 (20) | 2.2 | 1.9 (14) |
a
uninf., uninfected; inf., infected.
b
The fold induction at different time points was calculated by considering the secretion at 0 h to be 1-fold. Percent inhibition of TH2-related cytokine secretion was calculated by considering the levels or the percent induction in solvent-treated or sh-C-treated cells at the respective time points to be 100%. Boldface data indicate ∼2- or more than 2-fold induction. MK sol. treat., MK866 solvent treatment; MK treat., MK866 treatment; Zil. sol. treat., zileuton solvent treatment; Zil. treat., zileuton treatment.