FIG 3.

Titration of virus replication and luminescence expression for SINV expression viruses over 10 passages in BHK-21 cells. BHK-21 cells were infected with an MOI of 0.1 PFU/cell (P1) and then incubated for 18 h. Supernatants were then harvested and diluted 1:1,000, followed by infection of fresh cells. This was continued for 10 passages (P1 to P10). During each passage, infected cells or supernatants were harvested for plaque titration (right y axis) or in vitro luciferase assay (left y axis) as described in Materials and Methods. (A) TaV-fLuc; (B) nsP3-fLuc; (C) DP-fLuc; (D) TaV-nLuc; (E) nsP3-nLuc; (F) DP-nLuc. Error bars indicate standard deviations.