FIG 2.

Reduced formation of “mature” viral replication compartments (RCs) in XPE fibroblasts. Normal (BJ) and XPE dermal fibroblasts were infected at an MOI of 0.1. Cells were fixed each day p.i., and IE1 and IE2 proteins and pUL44 were detected by immunostaining. (A) Localization of IE proteins and pUL44. Immunofluorescent images of normal (BJ) and XPE dermal fibroblasts infected with HCMV. Cells with “immature” RCs were defined as those with multiple, small pUL44 compartments (yellow arrows), and cells with “mature” RCs were identified as those composed of single, larger pUL44 compartments (white arrows). 4′,6-Diamidino-2-phenylindole (DAPI) staining was used to define nuclei. (B) The percentage of fibroblasts with mature RCs was plotted relative to those lacking or having immature RCs. More than 200 cells were scored per sample. Mean values are shown, with bars denoting 1 standard deviation, for three independent experiments. For certain data points, error bars may be too tight to be visible.