FIG 7.

DDB2 and ATM function in the same genetic pathway to influence HCMV replication. (A) Viral replication when ATM is depleted in BJ and XPE fibroblasts. BJ and XPE fibroblasts were transfected with siRNAs specific for ATM (siATM) or with a control siRNA (siCON) 24 h prior to infection with HCMV at an MOI of 0.1. Infected cell supernatants were assayed for infectious virus production by plaque assay. The mean values are shown, with bars denoting 1 standard deviation, for three independent experiments. For certain data points, error bars may be too tight to be visible. (B) Transient depletion of ATM in BJ and XPE fibroblasts. BJ and XPE fibroblasts were transfected with siATM or siCON and infected with HCMV at an MOI of 0.1. The levels of ATM protein expression were assessed by immunoblot analysis.