FIG 3.

Digestion and immunoprecipitation products as sequenced by liquid chromatography-MS/MS. An example of a typical mass spectrometry result with detected peptides aligned with the primary amino acid sequence of the capsid protein of strain AU08 (N terminus to C terminus) is shown. Amino acids making up the protein sequence are indicated by one-letter identifiers, and amino acid position is indicated above the sequence every 10 residues. The large gray bars below the amino acid sequence indicate that a peptide was detected that matches the expected mass of a peptide with that sequence composition. The small dark gray boxes correspond to the identified residue masses as determined through MS/MS sequencing. A greater number of dark gray boxes indicates greater result certainty. (A) Peptides detected following digestion of the VLP, prior to immunoprecipitation. (B) Peptides detected following immunoprecipitation of digested VLP with rabbit anti-rAU08 serum. (C) Peptides detected following immunoprecipitation of digested VLP with rabbit preimmune serum.