TABLE 1.
Minimization of P2 domain immunoreactive regions using rabbit anti-rAU08 polyclonal serum and Mimotope-supplied peptides
Name and number designated to each Mimotope peptide (MP). The P2 domain immunoreactive region for which the Mimotope was designed is indicated in parentheses, where “(IR-4)” indicates aa 362 to 379 and “(IR-5)” indicates aa 380 to 405.
Site no. refers to amino acid residue number of the capsid protein according to GII.3 strain AU08 numbering.
For immunoreactive region IR-4 (aa 362 to 379), bold residues represent residues that are required to form the high-affinity epitope, based on ELISA results. Underlined residues represent those that likely form a second epitope with reduced binding affinity, based on ELISA results and homology modeling. Structurally internal residues that are likely important for epitope structural formation are italicized.
For immunoreactive region IR-5 (aa 380 to 405), residues forming the minimal binding region of the epitope are underlined and the residues that are essential to binding are in bold.
CP is the negative-control peptide (CP) supplied by Mimotopes.