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Growth kinetics of recombinant viruses in cells. A549, MDCK, and PK-15 cells were infected with the WT or variant viruses at an MOI of 0.001. At the indicated hours postinfection (hpi), the virus titers in the supernatant were determined with the MDCK cells. The reported values are presented as the means ± standard deviations of three independent experiments. **, P < 0.001, as determined by a t test.
