FIG 1.

Detection of viral proteins in cell lysates and supernatants. Western blots of lysates and concentrated supernatant of 293FT cells transfected with different plasmids. (A) Transfection of empty plasmid, pI.18/ANDV-GPC, or cotransfection of pI.18/ANDV-GPC and pCMV-Bios/ANDV-N. (B) Transfection of empty plasmid or pWRG/PUU-M(s2). ANDV Gn and N proteins were detected with anti-Gn 6B9/F5 and anti-N 7B3/F7, respectively. ANDV and PUUV Gc was detected with MAb anti-Gc 2H4/F6. No MAb against PUUV Gn was available.