FIG 3.

Reproducibility of fusion measurements. (A) Triplicate infections were performed with the same viral preparation with MDDCs or CD4 T cells. Error bars represent standard deviations. (B) Donor-to-donor target cell variability. Results of experiments in which the same viral preparations were used to infect the cells of two donors are presented. To account for changes in HIV-1 susceptibility, the fusion ratio corresponding to the percentage of cells supporting infection was divided by the fusion measurement for 81A. (C) Variability due to different plasmid preparation, virion production, quantification, and infection. For four genotypes (Env 1 to Env 4), two or three clones (represented by different symbols) were tested simultaneously using the same donor target cells. Note the similarity of the fusion phenotypes for identical genotypes.