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. 2014 Feb;88(3):1591–1603. doi: 10.1128/JVI.01812-13

FIG 2.

FIG 2

Detection of BEFV α1 and BEFV N in uninfected and BEFV-infected BHK-BSR cells. (A to C) Immunofluorescence images of uninfected (A), BEFV CS1865-infected (B), and BEFV CS919-infected (C) cells at 24 hpi. Frames illustrate differential interference contrast (DIC) images, cells stained with DAPI (blue) to highlight nuclei, cells stained with 1:500 dilutions of anti-BEFV α1 polyclonal rabbit serum (BEFV α1; green) or anti-BEFV N mouse monoclonal antibody 11A3 (BEFV N; red), and the merged fluorescence image (Merge). (D) Western blot analysis demonstrating the detection of BEFV N protein and BEFV α1 protein in BEFV-infected BHK-BSR cells at 1, 15, 24, and 40 hpi. Results for uninfected cells (mock) are also shown.