FIG 5.

Sense and antisense transcription at the EBNA2 locus (A) RNA-seq read coverage from poly(A) selected and ribodepleted RNA, and coding potential evidence summary from the Coding Potential Calculator. Note the inset with a zoomed scale for the positive (sense) strand in ribodepleted coverage. (B) RPKM values for RNA-seq time course from ribodepleted RNA (C) Relative abundance of antisense transcription from 0 to 48 h measured by strand-specific qRT-PCR. Primer placement is shown in panel A. (D) Relative abundance of antisense transcription at 24 h after treatment with anti-IgG, anti-IgG plus PAA, or nothing (control). Measured by strand-specific qRT-PCR. (E) Relative abundance of antisense transcription in the nucleus and cytoplasm at 24 h after treatment with anti-IgG. Measured by strand-specific qRT-PCR. (F) FISH of EBNA2 antisense transcripts.