FIG 2.

Serum cytokine and chemokine concentrations of rhesus monkeys following poxvirus vector boosting. Rhesus monkeys (n = 12/group) were primed i.m. with 3 × 10¹⁰ viral particles of Ad26 expressing SIV Gag, Pol, and Env and then boosted i.m. with 1 × 10⁸ PFU of the ALVAC, MVA, or NYVAC vector expressing transgenes. Sera were collected on days 0, 1, 3, 7, and 14 following vaccination, and systemic levels of cytokines and chemokines were analyzed by Luminex assays and ELISAs. (A) Systemic levels of selected cytokines and chemokines following boosting with the various poxvirus vectors, with individual levels shown as colored lines and group means over time shown as black lines. (B) Mean fold induction of all cytokines and chemokines in rhesus monkeys at day 1 postboosting with poxvirus vectors. The values shown are group mean fold induction over the averaged group baseline prior to boosting with the various poxvirus vectors.