FIG 3.

Induction of cytokines and chemokines by poxvirus vector stimulation of human PBMC. (A) Fresh whole human PBMC (n = 4/group) were stimulated at an MOI of 10 PFU/cell with the ALVAC, MVA, or NYVAC vector expressing no transgene. Cytokine and chemokines were measured 24 h postinfection by Luminex assays. Lipopolysaccharide (LPS; 1 ng/ml) was included as the positive control. The data are means and standard errors of the means. Bars indicate P values of <0.05 by Kruskal-Wallis tests. (B) Mean fold induction of all cytokines and chemokines at 24 h postinfection of human PBMC with poxvirus vectors. The values shown are group mean fold induction over the averaged group baseline of the unstimulated PBMC control.