FIG 4.

Cytokine and chemokine responses elicited by ALVAC in human PBMC depleted of various cell populations. Fresh whole human PBMC (n = 2 to 6/group) were depleted of the indicated PBMC populations, and depletion was confirmed by flow cytometry (data not shown). Unseparated and depleted cell populations were stimulated at an MOI of 10 PFU/cell with ALVAC, MVA, or NYVAC, and cytokine and chemokine responses were measured by Luminex assays at 24 h postinfection. The data shown are means and standard errors of the means. Lines indicate P < 0.01 by Kruskal-Wallis tests.