FIG 8.

Dose-response blocking of H77, J6, and S52 with and without HVR1 with LDLr-specific monoclonal antibody 3D8. Huh7.5 cells were plated in 96-well plates. The following day, the indicated concentrations of blocking antibody or a single high dose of control antibody was incubated with the cells for 1 h at 37°C (each concentration in three replicates), including 6 wells without antibody. Following 3 h of infection, washing, and an additional 45 h of incubation, the plates were stained with 9E10 antibody for H77 and H77_{ΔHVR1/N476D/S733F} (A), J6 and J6_ΔHVR1 (B), or S52 and S52_ΔHVR1/A369V (C). Blocking data are shown as dose-response curves with the means and SD of three replicates at the given antibody dilution normalized to 6 replicates of virus only. The open symbols represent data for the control antibody only tested at the highest antibody concentration. Four-parameter nonlinear curve regression was used to fit a curve to the data points (Graphpad Prism, v4.03). Z-tests were used to compare the differences in maximum attained blocking. ** and ***, statistical significance at P values of < 0.01 and <0.001, respectively.