FIG 1.

(A) Schematic representation of the organization of the VSV P protein domain in light of recent structural studies (16–18). The P protein has been divided into three domains, an N-terminal domain (P_NTD), an autonomously folded central domain (P_CD), and a negatively charged C-terminal domain (P_CTD). Previously reported phosphorylation sites and surrounding residues are shown. (B) Protein profile of VSV-infected cells. BHK 21 cells either mock infected or infected with VSV were metabolically labeled with [³⁵S]methionine-cysteine. Cell lysates were resolved by low-bis SDS-PAGE (see Materials and Methods). (C) P protein was isolated from VSV-infected BHK 21 cells by immunoaffinity purification (see Materials and Methods). N protein was copurified with P as shown. (D) The identities of the P and N proteins were determined by Western blotting analyses. The values between the blots are molecular sizes in kilodaltons.