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. 2014 Feb;88(3):1787–1794. doi: 10.1128/JVI.03227-13

FIG 4.

FIG 4

Attachment kinetics and eclipse kinetics of virion production of the wild type and mutants at the Q24 site. (A) Attachment kinetics of the wild type (black) and cs(H)G24Q mutant (blue) at 37°C (open circle) and 28°C (filled circle) and of sp(H)G24Y mutant (magenta) kinetics at 37°C (this mutant was not analyzed at 28°C). (B) Eclipse kinetics of preattached wild-type (black) and cs(H)G24Q (blue) particles at 28°C. (C) Virions isolated from wild-type (black)- and cs(H)G24Q mutant (blue)-infected cells at 37°C (filled circles) and 28°C (open circles). Gradients were fractionated from the bottom of the tube. Thus, S value decreases with higher fraction numbers. Particles, regardless of infectivity, were detected by UV spectroscopy.