TABLE 2.
Plating efficiencya of missense mutants
| Mutationb | Plating efficiency at indicated temp |
||||
|---|---|---|---|---|---|
| No plasmid |
pøXH |
||||
| 28°C | 33°C | 42°C | 28°C | 42°C | |
| ts(H)G3Q | 1.0 | 1.0 | 10−4 | 1.0 | 1.0 |
| ts(H)G8Y | 10−1 | 1.0 | 10−6 | 1.0 | 1.0 |
| cs(H)G24Q | 10−6 | 1.0 | 1.0 | 10−6 | 1.0 |
a
Plating efficiency is defined as titer on mutant with no exogenous H gene expression/titer on host expressing a cloned wild-type H gene at 33°C.
b
All mutant stocks were grown in the absence of exogenous H gene expression. Thus, plaque formation assays the ability of the mutant protein to infect.