FIG 3.

Antigenic profiles of a selection of Australian BEFV isolates (1956 to 1992) and associated amino acid substitutions. Virus neutralization was conducted in C6/36 cells using neutralizing monoclonal antibodies corresponding to antigenic sites G1, G2, G3a, G3b, and G4 (A to H), nonneutralizing monoclonal antibody (I), BEFV polyclonal rabbit antiserum (J), and uninfected cell culture fluid (K) (Table 1). Shaded boxes indicate positive neutralization as determined by the absence of BEFV-specific immunofluorescence. Amino acid substitutions mapping to the known antigenic sites are shown highlighting those associated with highly variable amino acids K²¹⁵ and R²¹⁸. Two of the isolates analyzed in this assay have not been sequenced (ns).