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. 2014 Feb;88(3):1421–1432. doi: 10.1128/JVI.03017-13

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Copyright © 2014 Ross-Thriepland and Harris.

This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

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FIG 8 — Phosphorylated S222 is predominately in the hyperphosphorylated species. A phospho-specific antibody was raised in sheep against pS222 using an appropriate 13-mer peptide and purified by IgG enrichment. (A) Specificity of the pS222 antibody. Lysates from Huh7 cells electroporated with the indicated subgenomic replicons were analyzed by SDS-PAGE/Western blotting and probed with either anti-pS222 (left panel), or anti-NS5A (right panel) sheep polyclonal antisera. (B) Indicated lysates were probed with the anti-pS222 antiserum. (C) Lysates were probed with anti-NS5A (upper panel) or anti-pS222 (lower panel).