TABLE 1.

NS5A phosphopeptides identified by mass spectrometry

Peptide fragment observed by mass spectrometrya	No. of phosphorylation events; location(s)b	[M+H]+		Type of MS analysis used
		Calculated	Observed	MALDI	ESI	MS/MS
KAPpTPPPR	1; T348	943.4761	943.4575	Yes	No	Yes
IPCQLPpSPE	1; S146	1120.4743	1120.4482	Yes	Yes	Yes
GpSPPSEASSSVSQLSAPSLR	1; S222	2023.9332	2023.9508	Yes	Yes	Yes
GpSPPpSEASSSVSQLSAPSLR	2; SS222/225	2103.8995	2103.8706	Yes	Yes	Yes
GSPPSEASSSVSQLSAPSLR	3; multiple isomers	2183.8659	2183.8477	Yes	No	Yes
GSPPSEASSSVSQLSAPSLR	4; multiple isomers	2263.8322	2263.811	Yes	No	Yes
GSPPSEASSSVSQLSAPSLR	5; multiple isomers	2343.7985	2343.7756	Yes	No	Yes
GSPPSEASSSVSQLSAPSLR	6; unassigned	2423.7648	2423.7419	Yes	No	No
GSPPSEASSSVSQLSAPSLR	7; unassigned	2503.7311	2503.7082	Yes	No	No
ATCTTHSNTYDVDMVDANLLMEGGVAQTEPESR	1; T242 or T244 or T245 or S247 or T249	3724.5449	3724.5441	No	Yes	Yes
TFGQPPSSGDAGSSTGAGAAE	1; S387 or S388	1931.7655	1931.7629	No	Yes	Yes
SGGPTSPGEPAPSE	1; S396 or T400 or S401	1349.5257	1349.5414	Yes	No	Yes

^aBold and underlining in sequences indicate phosphorylated residues and putative phosphorylated residues respectively.

^bJFH-1 NS5A protein numbering.