FIG 12.

Inhibition of LCMV infection by PALA. Subconfluent monolayers of A549 (A) and BHK-21 (B) cells were infected with LCMV (MOI, 0.01) and treated with the indicated concentrations of PALA. Aliquots of TCS were obtained at the indicated times postinfection and titrated (results in FFU/ml) by immunofluorescence. Viral infections were performed in triplicates. Dotted lines indicate the limits of detection for the assay. Mean values and standard deviations are shown. Statistical analyses were conducted using Student's t test within Microsoft Excel. *, P < 0.05. PALA cytotoxicity in A549 (C) and BHK-21 (D) cells was evaluated as described for Fig. 3. Cell viability was normalized to DMSO-treated cells (set as 100%). Mean values and standard deviations of three independent assays are shown.