FIG 6.

A3 exhibits a dose-dependent inhibitory effect on the activities of both the LCMV and Candid#1 minigenomes. BHK-21 cells in 12-well plates were transfected with LCMV (A) or Candid#1 (B) MG systems. At 5 h posttransfection, transfection medium was replaced with fresh medium containing the indicated concentrations of A3 (A and B, i graphs) or ribavirin (A and B, ii graphs). At 72 h posttransfection, cell lysates were prepared to determine CAT expression levels in an ELISA. Values correspond to mean values and standard deviations of two independent experiments. For each experiment, each sample was examined in duplicate.