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A3 exhibits a dose-dependent inhibitory effect on the activity of the LASV minigenome. 293T cells in 12-well plates were transfected with the components of the LCMV or LASV T7 MG systems. At 5 h posttransfection, transfection medium was replaced with fresh medium containing the indicated concentrations of A3. At 60 h posttransfection, cell lysates were prepared to determine CAT expression levels in an ELISA.
