FIG 3.

Molecular analysis of CDVd and CTV in (A) etrog citron and (B) Mexican lime. (Row 1) Circular forms of CDVd were tested by Northern blot hybridization using a DIG-labeled CDVd-specific probe in plants singly inoculated with CDVd and coinoculated with CDVd and CTV. (Row 2) CDVd-sRNAs were detected by Northern blot hybridization using a DIG-labeled CDVd-specific probe (etrog citron) or an α-UTP-radiolabeled riboprobe (Mexican lime). (Row 3) In all instances, RNA levels (5S and 4S RNAs) were comparable. (Row 4) CTV infection was confirmed by tissue-print ELISA using monoclonal antibodies 3DF1 and 3CA5.