FIG 4.

Molecular analysis of CDVd and CTV in wild-type Mexican lime singly infected with CDVd or coinfected with CTV and CDVd and transgenic Mexican lime lines expressing p23, p20, and p25 singly infected with CDVd. (Row 1) Circular forms of CDVd were tested by Northern blot hybridization using a DIG-labeled CDVd-specific probe. (Row 2) CDVd-sRNAs were detected by Northern blot hybridization using a α-UTP-radiolabeled riboprobe. (Row 3) In all instances, RNA levels (5S and 4S RNAs) were comparable. (Row 4) CTV infection was confirmed by tissue-print ELISA using monoclonal antibodies 3DF1 and 3CA5.