FIG 1.

SDS-PAGE of T5 protein components. (A) T5 ghosts were obtained from T5st0. Mature heads were produced from T5D17am34d, empty proheads II were produced from T5amN5, and tails were produced from T5D20am30d. Protein bands are listed by their number according to Table 2. The asterisk indicates the presence of the outer membrane major proteins OmpC and OmpF from contaminating host cell membrane vesicles. (B) (a) Western blot detection of T5p132 inT5st0 and T5hd1. Phage particles (≈50 μl, 10¹¹ PFU/ml, disrupted by repeated cycles of freezing and thawing) were resolved by SDS-PAGE and transferred to a nitrocellulose membrane for Western blotting. T5p132 protein was visualized by using rabbit anti-p132 IgGs and goat secondary rabbit antibodies (IRDye 800CW; Odyssey). (b) SDS-PAGE of T5hd1 proteins showing the absence of the L-shaped fiber protein pb1. MW, protein molecular weight markers in kDa.