TABLE 1.

Nucleotide sequence of T5 mutations affecting T5 assembly^a

Name	Gene/protein affected	Phenotype	Mutation	Stop codon positionb	Reference
T5D17am34d	D17 (T5p133), tail protein pb4	Filled mature head	C268T	90/689	34
T5D20am30d	D20 (T5p145), major head protein pb8	Functional tail	C1132T	378/459	34
T5amN5	T5p151, terminase large subunit	Empty prohead II	C1189T	397/439	8
T5amHA911	T5p149, nicking endonuclease	Nick-less DNA	C337T	118/151	43
T5hd1	ltf, L-shaped fiber protein	High density	+T873	304/1396	13

^aMapping of the mutations was determined by sequencing the late gene region of each T5 mutant genome: 6-kbp fragments amplified by PCR from purified T5 mutant DNA were sequenced using primers designed from the T5st0 genome sequence. In amber mutants, the Stop codon results from a point mutation. In T5hd1, the Stop codon results from an insertion (+) that alters the normal reading frame.

^bPosition of the translation termination relative to the total number of amino acids of the wild-type protein.