FIG 2.

Production of infectious virus by VPA treatment of infected cells. Infected Vero rKSHV.294 cells were treated with 1 mM VPA or 2 μM TSA, and infectious virus in the cell medium was quantitated by (A), SeAP assay from serially infected 293 MSR tet-OFF cells, as described in Materials and Methods. SeAP activity was normalized to that for negative-control cells, which had been incubated with supernatants from Vero rKSHV.294 cells treated with solvent only (mock). Alternatively, (B) virion-associated DNA (vDNA) was purified from triplicate infected cultures, and equal volumes were quantitated by dot blotting using an Rta-specific probe.