FIG 6.
VPA increases euchromatin markers and decreases heterochromatin markers on the ORF50/Rta promoter. ChIP assays were performed with BC-3 cells using antibodies to various modifications of histone H3 (as shown) and rabbit IgG (negative control). Fold changes were calculated using the ΔΔCT method and represent the change in ChIP of each H3 modification relative to the ChIP of IgG in the presence or absence of the inducing chemical. Numbers in parentheses refer to the base pair positions relative to the ORF50 transcription start site. Numbers in brackets refer to the base pair positions relative to the translational start sites.