TABLE 2.
HDACi-specific reactivation of KSHV from latency
| HDACi | Concn |
% maximum reactivation (SD)c | HDAC class specificity | |
|---|---|---|---|---|
| Range testeda | Optimumb | |||
| HC toxin | 2.0–10.0 nM | 10.0 nM | 2.3 (0.1) | I |
| VPA | 0.5–5.0 mM | 1.0 mM | 73.5 (1.4) | I, IIa, IIb (except HDAC 6) |
| TSA | 0.1–2.0 μM | 2.0 μM | 7.8 (0.8) | I, IIb |
| MC 1658 | 1.0–5.0 μM | 5.0 μM | 0.7 (0.1) | IIa |
| Nicotinamide | 100.0–900.0 μM | 300.0 μM | 0.3 (0.0) | III |
| Sirtinol | 5.0–500.0 μM | 50.0 μM | 0.5 (0.2) | III |
| Mock | 0.2 (0.0) | |||
a
HDACis were added to the medium of duplicate cultures of 3 × 105 Vero-rKSHV.219 cells in 35-mm dishes cells at the indicated concentrations. Reactivation was scored by quantitating the percentages of GFP-positive (total) cells that were also RFP positive (in which the E gene was induced) at 0 to 10 days following addition of the HDACis.
b
Optimal HDACi concentrations.
c
Maximum reactivation efficiencies.