FIG 2.

Mutations in E8 increase HPV16 episome copy numbers in normal human keratinocytes. (A) Southern blot analysis of low-molecular-weight DNA isolated from two different sets of low-passage-number HPV16 wt (wt) HPV16 E8− (E8−) or HPV16 E8 KWK mt (E8 KWK mt) cell lines. DNA was digested with XhoI, transferred to a membrane, and detected by hybridizing with a ³²P-labeled HPV16 genomic probe. As a size marker, 100 pg of linearized HPV16 wt genome was used (M). (B) Quantitation of HPV16 copy numbers by qPCR. Equal amounts (50 ng) of total genomic DNA from the different cell lines were analyzed by multiplex qPCR. The values represent HPV16 E2 copies per 2 beta-actin copies. Data are derived from four independent DNA preparations for each cell line. Error bars indicate the standard errors of the means.